#lustwithlife
Menu
0 Comments

RECENT ADVANCES IN GENETIC MANIPULATION IN THE TREATMENT OF CONGENITAL DEAFNESS

  • Attempt to reverse certain types of defect that affect the early development of the auditory and vestbular systems
  • Target group for screening: premature newborns and strong family history
  • Technique to locate the gene on particular chromosome or the mutation within the gene and the gene product.

GENE TRANSFER:

  • Attractive technology for clinical applications
  • It offers the hope for preventing, arresting , reversing or curing vestibular or hearing disorders caused by heriditary disease or environmental insults.
  • In utero gene transfer has many technical and ethical issues.
  • Vectors successfully studied are:
  1. cationinc liposomes
  2. adeno associated virus
  3. adeno virus
  4. lenti virus
  5. herpes simplex virus and
  6. vaccinia virus

VECTOR DELIVERY SYSTEM:

  1. Systemic administration: it has a disadvantage of systemic toxicity includng viral hepatitis and enchepalitis.
  2. Direct instillation:
  • Allows high vector concentration delivery to target organ
  • Needs small amount of vector solution
  • Increase likelyhood of gene delivery to target tissue
  • Minimizes leakage of vector to the surrounding.

Several techniques of intracochlear vector delivery are:

  1. Miniosmotic pump infusion or microinjection into scala tympani via round window
  2. Infusion or microinjection into the scala tympani through cochleostomy
  3. Injection into endolymphatic sac accessed from posterior fossa and
  4. Gel foam application to round window membrane.

Transgene expression in contralateral, noninjected cochlea occurs. Three potential routes of spread from cochlea have been suggested:

  1. Viral spread via cochlear aqueduct ehich connects scala tympani of basal turn of the cochlea to the CSF space
  2. Transmission of vectors through the temporal bone marrow space
  3. Haematogenous dissemination

STEM CELL AND GENE THERAPY:

  • Twin technology
  • Focus on regenrationg cochlear hair cells which are fuctioning by reversing the genetic defect as well

Leave a Reply

Your email address will not be published. Required fields are marked *

error: Dr. R Content is Protected !!!
%d bloggers like this:
Skip to toolbar